Whooping cough tests look for evidence of Bordetella pertussis, the bacterium that causes whooping cough. The test method, the sample and when it was collected all affect how the result is interpreted.
A culture tries to grow the bacteria in the laboratory. A PCR, short for polymerase chain reaction, looks for genetic material from the bacteria. Both tests usually use material collected from the back of the nose.
An antibody test looks for the body's immune response in a blood sample. Antibody tests have different limits, and some are used only in selected settings. The method named on the report therefore matters.
Culture tends to work best early in the illness. PCR can remain useful later, but detection becomes less likely as time passes. Antibiotics taken before testing and a sample that did not collect enough material can also reduce the chance of finding evidence of the bacterium.
A positive PCR result is interpreted together with symptoms and possible exposure. A negative result does not always rule out whooping cough.
The report should be read with the test method and collection date. The result alone does not show when the infection began or replace the clinical assessment.

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